ABSTRACT
The aim of the present study was to evaluate the antiproliferative activity of crude extracts from leaves of Bauhinia variegata and Leptadenia reticulata using fission yeast Schizosaccharomyces pombe as a model. The crude extracts of both plants were prepared by three experimental conditions (25°C, elevated temperature and ultrasound waves) and four solvent systems [Water, Methanol, Water: Methanol (1:1) and Chloroform: Methanol (1:1)]. MTT assay was performed for estimating the cell viability and the results were expressed as % growth inhibition. Overall based on the MTT assay performed on yeast cells, Water: Methanol, Methanol and Water extracts showed higher antiproliferative activity than the positive control (Paclitaxel) in a dose-dependent manner. For Bauhinia variegata and Leptadenia reticulata, methanol extract (25°C temperature & elevated temperature, respectively) exhibited highest inhibition which was approximately 80%. In conclusion, this study has indicated that Bauhinia variegata and Leptadenia reticulata extracts possess strong antiproliferative activity. Furthermore, it could act as a potential source for identifying new molecules with chemotherapeutic potential against cancer.